
The above graph gives the absorbance of vitamin B, solution in water, in a 1.00 cm...
1. Did the column separate NADH and BSA? Explain based on your
graph
2. What assumptions have you made in determining the
absorbance of the mixture at two different
wavelengths?
3. Is the reaction catalysed by GPT a reversible
reaction? Give evidence from your experimental results in Table 3
to support your answer. (I DONT UNDERSTAND THIS QUESTION PLEASE
HELP)
Separation of BSA and NADH using Gel filtration chromatography 0.060 0.040 0.020 0.000 0.000 4.000 3.000 5.000 6.000 2.000 -0.020...