Question

If I could have the answer to all of these that would be great!

1. NMR researchers have an E. coli culture growing in a medium containing 15NH4C1. By accident, they switch to a medium containing 14NH4Cl. This culture environment continues for three generations (an 8-fold increase in population). What will be the molar ratio of hybrid DNA (1-N- 14N) to light DNA (14N-14N) at this point? Show how you came up with this ratio. 3 pts 2. In vertebrate cells, DNA cytosine if often methylated to form 5-methylcytosine. There is a specialized repair system that recognizes G-T mismatches and corrects them to G-C. Explain the connection between these different facts and why some end up being advantageous to the cell. 3 pt 3. What effect would absence of Mg2+ have on DNA polymerase activity? Defend your answer from protein chemistry perspectives.. 3 pts 4. One of the approved anti-HIV drugs is 2', 3'-dideoxyinosine. Explain how this drug might block DNA replication and thus growth of HIV 3 pts

This is the only info I was given

Answer 1

y This explanation is based on Semiconservative Principle of replication of DNA which was proved by meselson and stahl expr. when E. coli was grown in those medium then both the stand had in Cheavy isotope)

Hrull heneration on (normal) is whyce two hybrid 2:0 hybrid pure

heneration (2) Total twonybrid (H+) nolar natio Total two Pure (1 ) Pure : hybrid Rw

henenation 3 Two hybrid DNA (mt) Toyə gix Pro p19 A (1) molan natio Pure a hybaid 2
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