Question

Using the figure below, estimate the total column volume, Vt, for the SEC column with 10 nm particle size. (Note: the answer should have only one decimal). Picture1.png

Answer 1

Ang Imate the tatal Column valume, vi for the SEC column with lonn. particle. 100 pm lopm log (moleculan mass) 100nm Sonn lonn som 5.0 6.07,0 10.0 11.0 120.8.0 90 Elution volone (mt). In SEC the Column volum of substances (Polymers) depends on their sizes. Particle size and Column volume are inversely proportional to each other that is bigges sized particles have 1 smaller Column volumes and vice versa, there there the colum shown in the figure is packed with a mixture of materials of different pore sizes Crixed gels). These types of Columns used to separate samples having a molecular weight range extremely broad.

smallest pore size of the som and it is gradually! som to looo microme- The Columm is increase from -ters through lon, 50m, 100nm, I micrometes, lo micrometer, and 100 micrometes respectively. Now the smallest sized pore that is som one is used to separate small molecular weight of the Polymers having Particle size less than 5um. 100 micrometers one used for seperating Polynets having very large particle - sizes and so on. e st a How the sample to separate here have lon Particles size and the Columa have pore sizes of 5nom 10nm and sonm in the lower ranger in the low How torm Particle size can not.

enter in Som Pores, it is also difficult for the sample to enter into the pores having exactly same size otherwise there is ehigh possibility to stuk it in. inside. So the 10nm sized pore is ideal for the given Sample, How if the particle entes in to the 2 Sonm Pore of the column, it will have an elution volume of approximately. 9.5mL as it appeats from the figure. Therefore the estimated Column volume is 9.5 mL.