Question

Question 3 (2 points) The Mg- metal ions involved in the enolase reaction mechanism are required for which reason? They allow a Lys in the active site to donate a proton to the 2-phosphoglycerate substrate They prevent the reverse catalysis of 3-phosphoglycerate to 2-phosphoglycerate They stabilize the phosphate group of 2 phosphoglycerate they enhance the electron withdrawing potential of the carbonyl group of 2-phosphoglycerate. they form covalent bonds with a prophyrin ring to coordinate the substrate

Answer 1

Solution : First we draw the mechanism of Enolose reaction to under stand this problem. Ma Mg Poz- ------ 0 1 H Mgt? PO2- POR 0 Mitz C-C-C-H -o- H oH MC ZGC-H zho OH - H₂O INH HO HO HH c=0 Lyu 211 Glum Ly, 345 Gly?! (2-phosphogelycerate) on the basis of above reaction mechanism The we can required say 3 that - because 12 metal ious involved in the enolase reaction mechanism are withdrawing potential of the Carbonyl group of y "They enhance the electron 2- phosphoglycerate."