Question

2 5 centimeter SR-militer S . O millimeter 100 micrometer 2 gram nanjorams 2 20 Q.2. In the electrophoresis lab exercise of separating proteins on the basis of net charge, you used pH 8.6 buffer and the net charge of four different proteins is shown below. Predict how net charge will change if pH 7.5 or pH 6.0 bufer was used? Protein Isoelectric Point Net charge @pH 8.6 Net charge pH 7.5 Net charge pll 6 10.2 Positive 7.2 Negative Negative Very Negative

Answer 1

Iwet charge | Net charge Soepoint @ PH 8. 6 I @ PH 7.5 moltin O SITE net charge @ pH G I very positivel 10.2 Positive Pesinine CONSECUTIVE ALLA 7.2 Inerativ negative negative positive ILIUL LAGU I LAR CITY 6-8 negative Negative Positive op 4.8 very negative negative Negative.