Question

Diazepam tablets are supplied in 2, 5, and 10 mg increments. Suppose several tablets are received as evidence and using the Physician's Desk Reference (PDR) you are able to tentatively identify them as valium. Diazepam (valium) is a member of the benzodiazepam family of drugs and at one time was the most prescribed drug in the country. Is Diazepam acidic, basic or neutral? Justify your answer. Draw the structure, and indicate the ionization center What would be an optimal pH for extracting neutral (organic) diazepam from an aqueous mixture? Suppose further that you learn that the tablets contain anhydrous lactose, starches, dyes, and calcium stearate. Describe a method using lecture material for isolating the active ingredient from the fillers using an extraction scheme. Justify and explain each step of the proposed method.

Answer 1

Diazepam is basic in nature as there are two N - atom to accept proton & weak basic imine group. Structure: It has single ionization centre, i.e., the imine group. Method for extracting Diazepam and its major metabolites from goat serum suing Discovery DSC - 8 SPE 96 - Well plate: SPE: Discovery DSC - 8 SPE 96 - well plate (100 mg) well Condition & equilibrate each were with 1 ml methanol and 1 ml Dl H_2 O. Load 1 ml, 0.5 mu g/ml diazepam and metabolited spiked in goat serum diluted in 25 on M ammonium formate, pH 71 (1: 1, v/v): n = 3. Wash with 1 ml 20% methanol in 25 mM ammonium formate, pH 2.75. Elute with 1 ml 60% methanol in 25 mM ammonium formate, pH 2.75.