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Why is it a bad idea to use polystyrene cuvettes when making UV absorbance measurements in...

Why is it a bad idea to use polystyrene cuvettes when making UV absorbance measurements in the 200-300 nm range? Choose one.

a. Polystyrene will be attached by acid.

b. Polystyrene absorbs a lot of light in this range.

c. Polystyrene might react with some species in the sample.

d. Polystyrene fluoresces in this range.

e. Polystyrene will be attacked by some organic solvents.

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Answer #1

Polystyrene is transparent to visible light but as it is aromatic ( has conjugative double bond) directly absorb UV light in the range of 200 to 300 nm.

Hence, option b is the correct option.

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