Why ink must be avoded in marking TLC plates? Why is TLC analysis limited to nonvolatile...
Why ink must be avoded in marking TLC plates?
Why is TLC analysis limited to nonvolatile samples?
Homework Answers
ANSWER
1....INK matter expansion on sheet will cause unexpected value of orignal mixture componanets.
2...TLC is mainly use with nonvolatile samples..because volatile sample easily vaporized on room tepmerture ..
volatile substance not give color vision mark to identify mixture componants...
Thin-layer chromatography (TLC) is a chromatography technique used to separate non-volatile mixtures. Thin-layer chromatography is performed on a sheet of glass, plastic, or aluminium foil, which is coated with a thin layer of adsorbent material, usually silica gel, aluminium oxide (alumina), or cellulose. This layer of adsorbent is known as the stationary phase.
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Why ink must be avoded in marking TLC plates?
Why is TLC analysis limited to nonvolatile...
TLC Analysis Of Egg Lipids Lab 1. The TLC plates were pre-baked at >120C. Why is...
TLC Analysis Of Egg Lipids Lab 1. The TLC plates were pre-baked at >120C. Why is this step necessary? 2. Below is the structure of your lipid staining dye, Naphthol Blue-Black. Explain which noncovalent interactions would be most important in allowing the dye to adhere to lipids (consider the chemical property of lipids as well as the dye). 3. You ran a solution of a fatty acid and a wax on a TLC plate using the exact conditions used in...
Why must the spot applied to a TLC plate be above the level of the developing...
Why must the spot applied to a TLC plate be above the level of the developing solvent? What problem will ensue if the level of the developing liquid is higher than the applied spot in a TLC analysis?
11. Why should you use pencil instead of pen when marking on a thin layer chromatography...
11. Why should you use pencil instead of pen when marking on a thin layer chromatography plate? a. Pencil lead isn't as dark. b. The components of pen ink will separate along with your sample, while pencil lead will not. c. Pen ink will not mark on a TLC plate. d. Pen ink will undergo a chemical reaction with the solvent. 12. Which of the following is not a reason to use thin-layer chromatography? a. To determine the number of...
Why would fluorene bind to dichloromethane more than fluorenone in TLC plates when fluorene is a...
Why would fluorene bind to dichloromethane more than fluorenone in TLC plates when fluorene is a non-polar molecule and fluorenone is a polar molecule?
1. Why should any marks on the TLC y marks on the TLC plate be done...
1. Why should any marks on the TLC y marks on the TLC plate be done in pencil and not pen? 2. A spot on a TLC plat migrates 4.2 cm as the solvent plates my migrates 4.2 cm as the solvent plates migrates 8.0 cm up the plate. What is the Rivalue for this spot? Show your calculati
When observing the TLC plates, were all the spots nice small circles? If no, provide and...
When observing the TLC plates, were all the spots nice small circles? If no, provide and explanation as to why this might be the case. 3.
Why must Rf values be quoted along with the specific solvent system used for the TLC...
Why must Rf values be quoted along with the specific solvent system used for the TLC experiment concerned?
10. The following TLC plates have been spotted with two different compounds and are about to...
10. The following TLC plates have been spotted with two different compounds and are about to be eluted. For each plate, explain if it has been spotted correctly or incorrectly and why. (see attached) Question 10:
Which compound would have the higher Rf value- Cholesterol or Cholesterol Stearate? Explain why (TLC analysis,...
Which compound would have the higher Rf value- Cholesterol or Cholesterol Stearate? Explain why (TLC analysis, Polarity, etc).
1. On development of the TLC plates for ortho- and para-nitroaniline, which isomer travelled faster? Why?...
1. On development of the TLC plates for ortho- and para-nitroaniline, which isomer travelled faster? Why? What was the solvent? What is the thin layer made of? (4 pts) 2. 1.000 grams of acetone was reacted with 3.651 grams of benzaldehyde in a mixture of water and ethanol and an excess NaOH. The isolated dibenzalacetone product was 2.015 grams. What was the limiting factor? What is the structural formula of the product? What was the yield as percent of theoretical?...
1. Why should any marks on the TLC y marks on the TLC plate be done in pencil and not pen? 2. A spot on a TLC plat migrates 4.2 cm as the solvent plates my migrates 4.2 cm as the solvent plates migrates 8.0 cm up the plate. What is the Rivalue for this spot? Show your calculati
When observing the TLC plates, were all the spots nice small circles? If no, provide and explanation as to why this might be the case. 3.
10. The following TLC plates have been spotted with two different compounds and are about to be eluted. For each plate, explain if it has been spotted correctly or incorrectly and why. (see attached) Question 10:
1. On development of the TLC plates for ortho- and para-nitroaniline, which isomer travelled faster? Why? What was the solvent? What is the thin layer made of? (4 pts) 2. 1.000 grams of acetone was reacted with 3.651 grams of benzaldehyde in a mixture of water and ethanol and an excess NaOH. The isolated dibenzalacetone product was 2.015 grams. What was the limiting factor? What is the structural formula of the product? What was the yield as percent of theoretical?...
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