Question

In attempting to determine the composition of a nucleotide sequence, the chain terminating method employs the following type of terminator. Why does it work?

NH Linker HO-P0-P0-P-01 | H OH OH NH9 Dye Nucleotide = Linker between nucleotide and dye

		It is missing the 2' OH.
		The linker is too big to allow further transcription.
		It is missing the 5' OH.
		It is missing the 3' OH.
		It has a triphosphate instead of a monophosphate.

Answer 1

The correct answer is The linker is too big to allow further transcription. Due to this for the determination of composition of nucleotide sequence the chain terminator method is used.