Question

You have 42 nmoles of a lyophilized (dried) oligonucleotide ( a short stretch of DNA). How...

You have 42 nmoles of a lyophilized (dried) oligonucleotide ( a short stretch of DNA). How much volume in μL do you need to make a 100 μM solution?

0 0
Add a comment Improve this question
Know the answer?
Add Answer to:
You have 42 nmoles of a lyophilized (dried) oligonucleotide ( a short stretch of DNA). How...
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Not the answer you're looking for? Ask your own homework help question. Our experts will answer your question WITHIN MINUTES for Free.
Similar Homework Help Questions
  • 1- Calculate the number of grams needed to make 1 mL of a 1M solution of...

    1- Calculate the number of grams needed to make 1 mL of a 1M solution of the oligonucleotide (32nt) 2- You have a 300 μM solution of a primer (45nt) in a volume of 400μL, how many μg of primer do you have total? 3- You received a primer (25nt) which came to you as a powder with a mass of 0.31 mg a- what concentration of primer do you have when you add 100μL water? b- How would you...

  • After designig primers to target a gene of interest, you recieve 33.4 nmoles of a forward...

    After designig primers to target a gene of interest, you recieve 33.4 nmoles of a forward primer from the company. You want to make a 100uM stock but you accidentally made a 1mM stock solution. How much water do you need to add to your 1 mM stock to make it 100 uM?

  • Prepare a fragment of DNA to be cloned by PCR by preparing the oligonucleotides received through...

    Prepare a fragment of DNA to be cloned by PCR by preparing the oligonucleotides received through dilutions. The DNA to be used as a template is in a 50 ng/ul solution. The protocol is as follows: VOLUME TO ADD FINAL CONCENTRATION 0.5 UM 0.5 M REAGENT 10 PM Forward Primer 10 PM Reverse Primer Thermoestable pol Master mix 2x Template DNA water 1x 100 ng.. Total volume 25 ul Information about the primers: Forward primer: 29.3 nmoles - 220 ug...

  • You need to amplify a specific DNA sequence from a dried drop of blood at a...

    You need to amplify a specific DNA sequence from a dried drop of blood at a crime scene. A forensic hematologist informs you that there are approximately 1000 white blood cells and 5000 red blood cells in this dried sample. She also confirms that the sample is human blood. The DNA sequence you choose to amplify occurs once per haploid genome. If you perform 30 cycles of amplification in a thermal cycler using Taq polymerase, how many copies of the...

  • Question 5 Using stock solutions in a protocol: from volume to final concentration A DNA ligation...

    Question 5 Using stock solutions in a protocol: from volume to final concentration A DNA ligation reaction is carried out in a 25 μL reaction mix. This is a reaction carried out in cloning. You are going to do a number of these reactions so you decide to make up a larger volume of the reaction mixture, called a master mix. This saves on multiple repetitive pipetting and reduces errors. You have been given a protocol to make up 1...

  • You are preparing to clone a DNA fragment into a plasmid vector. You start by linearizing...

    You are preparing to clone a DNA fragment into a plasmid vector. You start by linearizing your plasmid (concentration = 500 ng/μl) with EcoRI, which is provided in a standard 50% glycerol solution at 10 units/μl. Your enzyme also comes with an appropriate 10X reaction buffer.  Taking into account the final allowable glycerol concentration, you want to add the maximum amount of EcoRI, to achieve complete digestion of 1 μg plasmid DNA in a total volume of 20 μl. Fill...

  • 1. How would you prepare 250mL’s of a 12% sodium citrate solution? 2. Your stock DNA...

    1. How would you prepare 250mL’s of a 12% sodium citrate solution? 2. Your stock DNA is at 850μg/ml. You want to make 100μl of a 50ng/μl stock, using TE as your solvent. How would you do this? 3. You have a 10mg/ml sucrose solution. How would you prepare 15ml of a 10 μg/ml sucrose solution? Not good at the math part of these questions, please help!

  • Question 2 Expressing concentration in different units You have a 0.2 M solution of a compound...

    Question 2 Expressing concentration in different units You have a 0.2 M solution of a compound (molecular weight 200) a) How many mmoles are there in the following volumes? i. 2 mL ii. 5 mL ii. 10 mL iv. 100 mL b) Express this concentration in the following units: i. mM; iii. iv. %(w/v); mg/dL; c) How much of this compound would you have to weigh out if you were to make up 100 mL of the solution? d) If...

  • 4. If you have DNA with a concentration of 2 μgǐl, how much DNA (in 1)...

    4. If you have DNA with a concentration of 2 μgǐl, how much DNA (in 1) must be added to make a 20 μ l solution with a DNA concentration of l pgul? Hint: ci X V,-C2 X V2

  • Question 7 Working with buffers You need 100 ml of 120 mM phosphate buffer and you have two stock...

    please hlep me answer those three questions asap. Please ignore question 7. Question 7 Working with buffers You need 100 ml of 120 mM phosphate buffer and you have two stock solutions from which you can make the buffer; 0.6 M NaH2PO4 (the acid form) and 0.6 M Na2HP04 (the base form). You need to add 3 times more of the base form than the acid form to achieve the desired pH. How will you make this solution? Question 8...

ADVERTISEMENT
Free Homework Help App
Download From Google Play
Scan Your Homework
to Get Instant Free Answers
Need Online Homework Help?
Ask a Question
Get Answers For Free
Most questions answered within 3 hours.
ADVERTISEMENT
ADVERTISEMENT