Question

1. You must prepare a solution to use as an environment that involves a particular strain of bacteria that has a very limited pH range in which it can survive. This bacteria needs a medium with a pH = 6.10. You are tasked with making 500 mL buffer for this bacterium given the following reagents available to you in the lab, these are in the table

b. State which reagent(s) and exactly how much you used of each to create the buffer!

Reagents

Please show relevant calculations.

d. Would your buffer you made guard better against acid or base? What is the change in pH (a) 100 mL of 0.1 M HCl was added to the buffer (b) 100 mL of 0.1 M NaOH was added to the buffer? MUST SHOW WORK!!!

                                          change in pH (a) 100 mL of 0.1 M HCl ____________________            

                                                change in pH (a) 100 mL of 0.1 M NaOH______________________                    

Would your buffer you made guard better against acid or base?_________________

Reagents	Ka	pKa
0.1 M Hydrochloric acid, HCl	1.3 x 106	- 6.11
0.1 M Nitric acid, HNO3	2.4 x 101	- 1.38
0.1 M Acetic acid, CH3COOH	1.7 x 10-5	4.77
0.1 M Sodium Acetate, CH3COONa
0.1 M Benzoic Acid, C6H5COOH	6.3 x 10-5	4.20
0.1 M Sodium Benzoate, C6H5COONa
0.1 M Carbonic acid,H2CO3	4.3 x 10-7	6.37
0.1 M NaHCO3-	4.8 x 10-11	10.32
Reagents	Kb	pKb
0.1 M Ammonia, NH3	1.8 x 10-5	4.74
0.1 M Ammonium Chloride, NH4Cl
0.1 M Methylamine, CH3NH2	4.4 x 10-4	3.36
0.1 M Methyl Ammonium Chloride, CH3NH3Cl
0.1 M Sodium Hydroixde, NaOH		- 20

                                         

Answer 1