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14Carbon label was detected in Carbon dioxide with 3 turns of citric acid cycle. Where was...

14Carbon label was detected in Carbon dioxide with 3 turns of citric acid cycle.

Where was the radiolable in acetyl Coenzyme A? why?

Where was the radiolable in pyruvate?why?

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Answer #1

There was no radiolabel in acetyl coenzyme A because it is formed by the decarboxylation of pyruvate, the carboxyl group of which actually contains radiolabel. The reaction is shown below.

CH3-CO-14COO- + NAD+ + H-CoA ----> CH3-CO-CoA + NADH + 14CO2

The carboxyl group of pryvate contains the radiolabel, as shown above.

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