Question

Which of the following statements regarding thin layer chromatography are TRUE? Partition chromatography separates compounds based on how they distribute themselves between the stationary and mobile phases. In thin layer chromatography, a polar organic compound will be attracted to the adsorbent and therefore travel further up the plate than a non-polar compound The Rf value is independent of the adsorbent material used. In thin layer chromatography, a polar organic compound will be repelled by the adsorbent and therefore travel not as far up the plate than a non-polar compound The solvent must cover the sample spots to obtain proper Rf values. Sample spots should be approximately 1 cm in diameter.  

Answer 1

Correct option:

Partition chromatography separates compounds based on how they distribute themselves between the stationary and mobile phases.

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In thin layer chromatography, a polar organic compound will be attracted to the adsorbent and therefore travel less up the plate than a non-polar compound.

The Rf value is dependent of the adsorbent material used.

In thin layer chromatography, a polar organic compound will be attracted by the adsorbent and therefore travel not as far up the plate than a non-polar compound.

The solvent must not cover the sample spots to obtain proper Rf values.

Sample spots should be approximately 1 - 2 mm in diameter.