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Be sure to answer all parts. Give the amino acid sequence of an octapeptide that contains...

Be sure to answer all parts.

Give the amino acid sequence of an octapeptide that contains the amino acids Gly, His, Thr, Lys, Arg, Asn (2 equiv) ,Ile, and forms the following fragments when partially hydrolyzed with HCl: His―Ile―Thr―Arg, Lys―Asn―His―Ile, and Gly―Asn―Lys.

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Answer #1

The given amino acid sequence on complete hydrolysis gives

Gly, His, Thr, Lys, Arg, Asn(2 equiv), Ile.

and partial hydrolysis gives

His-Ile- Thr-Arg , Lys-Asn-His-Ile, Gly-Asn-Lys.

Now the structure have to placed as two amino acid overlaps

As Asn is present in 2equiv then two fragment combined as

Theses combined to form.

this combined with other fragment to form the amino acid sequence

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