for HPLC list 3 parameters of the column that you could change if you were choosing...
for HPLC list 3 parameters of the column that you could change if you were choosing a whole new column to improve isocratic seperation of two peaks that were overlapping in hplc.
note that flow rate is not a property of the column
Homework Answers
ANSWER:-
you can improve overlapping peaks with these parameters, but also you should mention that what column you are using and what is the dimension of it ,anyway i am trying to explain from my side.
THREE PARAMETERS ARE:-
1)- injection volume.
2)- run time.
3)- % of acetonitrile in mobile phase
Increasing the injection volume will cause delay before you see your first peptide. It may be better to wait until the injection front is gone before you start a solvent gradient. It just means that you have to account for the time it takes for the injection volume before starting solvent system
Increasing run time - it all depends on what you mean by this question. If you want to increase the time, it may help resolve closely eluting peptides.
If we are indeed discussing Reverse Phase HPLC, increasing acetonitrile concentration may help elute very hydrophobic peptides and wash the column.
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for HPLC list 3 parameters of the column that you could change
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