Question

This question is about microscopy. Several of the cameras in the lab can capture a greyscale image either as an 8-bit image or as a 12-bit image. What would be the difference between them, and what would be the benefit of using one over the other? What would be the disadvantage? Note, in each case, there would be a single channel (i.e. no color).

Answer 1

solution:

Depending on the camera used, each pixel can carry from 1 to 32 -bits. For a normal 8-bit monochrome camera each pixel has a possible 256 grey scale values.

Black =0

white = 225

For a 12-bit monochrome  camera each pixel has a possible 4096 grey scale values

Black =0

White = 4095

The human eye can accurately detect around 40-60 grey levels. 8-bit camera detect 256 grey levels; each grey level captured will be accurately repeated by the monitor display . 12 -bit cameras generate 12-bit images; These contain 4096 grey levels . The 12-bit scale gives far greater ability to capture the Dynamic range. This allows you to extract your data from a larger range.

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