Question

1.- Mark has received a very expensive protein that may be critical in the discovery for cancer treatment. Before running his experiment, he remembers his protein’s concentration is labeled “50X”. Mark wishes to utilize a final volume of 200 µL.

1. By what dilution factor must Mark dilute his expensive protein to be in working conditions (1X)? How many parts of protein and buffer make up this dilution?

      B. How much volume (in µL) of protein and buffer would Mark have to add to arrive at his desired dilution? Show your work

Answer 1

!.

A.

Here, final concentration is = 1X and initial concentration is = 50X,

So, dilution factor is = 1/50

Now, a dilution factor of x/y means, there should be x part of solute and (y-x) part solvent. So, in this solution, there is 1 part protein and 49 part buffer.

B. According to condition of equivalency,

Here,

So, we have to take 4 microlitre of protein and (200-4)=196 microlitre of buffer to arrive at this dilution.