25-6 (a) Why are HPLC particles porous? (b) Why are particles with 60- to 120-Å pores...
25-6 (a) Why are HPLC particles porous?
(b) Why are particles with 60- to 120-Å pores used for small
molecules
but wide-pore 300-Å stationary phases used to separate
polypeptides
and proteins?
Homework Answers
a) Because they provide a high surface area
show good retention and sample capacity
provide a base for further bonding of organic molecules.
b) particles with 60 to 120 A° pores used for small molecules because of the separation of small molecules with molecular weight of less than 5000 approx. but wide pores are used so that large particles like proteins and peptides can separte because the large pores size restrict diffusion of these large molecules is not seen.
Add Answer to:
25-6 (a) Why are HPLC particles porous?
(b) Why are particles with 60- to 120-Å pores...
1.) Describe the goals of a Gel Filtration Chromotography Experiment??? 2.) Explain each key theoretical principle...
1.) Describe the goals of a Gel Filtration Chromotography
Experiment???
2.) Explain each key theoretical principle of a Gel Filtration
Chromotography, and how they help acheive the goal???.
4.) Explain the key equations used in the Gel Filtration
Chromotography experiment and the terms involved in the
equation????
HI im trying to prepare for a lab/report and i have some
questions i could use help with please :) over all having trouble
seeing how everything ties together etc :) thank you...
Problem 3 (25 p) A 120-V, 60-Hz, three-phase, Y-connected, 6 poles, wound-rotor, synchronous motor has a...
Problem 3 (25 p) A 120-V, 60-Hz, three-phase, Y-connected, 6 poles, wound-rotor, synchronous motor has a synchronous reactance of 3 ohms. The motor takes a current of 10 A at 0.8 pf leading when operating at a certain field current. The load is gradually increased until the motor develops its maximum torque while the field current is held constant. Determine: a) The new line current b) The power factor c) Torque developed by the motor
12. A slab on grade measures 120 long x 60" wide x 2' 6" thick. What is the cost to form the slab...
12. A slab on grade measures 120 long x 60" wide x 2' 6" thick. What is the cost to form the slab if formwork costs S1 per square foot of contact area? A. $72 B.S90 C.S162 D.S180 13. A place concrete' activity requires the placement of 2,080 cubic yards. If the individual production rate is 2 cubic yards per workhour per person, with a 10-person crew and 8-hour workday, how many days are needed to complete this activity? A....
180 140 100 200 160 120 80 60 40 20 11 10 9 6 pm 7 3 2 ppm 100- 80- 60- 40- 20- 75 100 125 150 25 50 m/z Relative...
180 140 100 200 160 120 80 60 40 20 11 10 9 6 pm 7 3 2 ppm 100- 80- 60- 40- 20- 75 100 125 150 25 50 m/z Relative Intensity o Write all of your notes on the spectra Type a justification of each structure in the same order they appear in your stapled packet o Insert page breaks so each structure is on a separate page o Include page numbers o Type your name in the...
QUESTION: DATA FOR QUESTION: 60 b. You have determined that ABC would be a more effective...
QUESTION:
DATA FOR QUESTION:
60 b. You have determined that ABC would be a more effective costing method. You have decided to 61 split the costs into 3 activities: Routine trip, maintenance, and machine lease-space rent-utilities. 62 Develop an analysis to determine the activity rates for each of the 3 activities 63 Round answers to the nearest cent. Routine Trips Machine lease, space rent, and utilities Maintenance Supervisor Salary Head cashier Salary Associate cashiers Salary ($1,800 each Assoc. cashier) Maintenance...
Product A Product B Selling Price $25 $20.40 Direct Materials($2/lb) 4.00 6.00 Direct Labor($6/hr) 6.00 3.00...
Product A Product B Selling Price $25 $20.40 Direct Materials($2/lb) 4.00 6.00 Direct Labor($6/hr) 6.00 3.00 Variable Overhead 2.00 .80 Fixed Overhead 1.60 1.60 Variable selling expense 1.00 1.00 Fixed Selling/administrative Expense 2.40 17.00 2.40 14.80 Net Income $8.00 $5.60 The company has a maximum 1,000 labor hours available for the upcoming period. This represents the only internal constraint on capacity. The company keeps no finished goods inventory and currently estimates a maximum demand of 350 units of Product A...
1. Like phospholipids, lipopolysaccharides have a hydrophilic head and a hydrophobic tail. True or false 2....
1. Like phospholipids, lipopolysaccharides have a hydrophilic head and a hydrophobic tail. True or false 2. The outer membrane of Gram negative bacteria contains lipopolysaccharides T or F 3. Biofilms and pure culture are formed by only one type of microorganism T or F 4. Any given antibiotic will have the same minimum inhibitory concentration (MIC) against any test microorganism. T or F 5. Production of antibiotics by the microorganism is not affected by the culture conditions T or F...
En (2 points) You isolated your mitochondrial DNA in Part I. In step 6, you discard...
En (2 points) You isolated your mitochondrial DNA in Part I. In step 6, you discard the supernatant, but keep the pellet. In step 15, you discard the pellet, but keep the supernatant. Explain why the pattern is different between the two steps and the consequence of mixing up these two steps. Procedure Part 1: mt DNA Isolation from your cheek cells. Lysis solution is used to breakdown the cells in this step, you will isolate MEONA from cheek cells....
1. Which of the following is not considered a nutrient a Vitamin B. Water c.Carbohydrate (d)...
1. Which of the following is not considered a nutrient a Vitamin B. Water c.Carbohydrate (d) Alcohol e Mineral 2. For which of the following causes of death does a person's diet play a part? a. Lung disease (6) Cancer Chronic lower respiratory diseases d. Infections of the blood e AIDS 3. Researchers repeatedly report that people who consume a variety of foods such as fruits, vegetables, legumes, nuts, and whole grains have reduced risks of which of the following...
3.2 Periodic trends 1. (0620-5 2012-Paper 1/2-Q21) Which properties of the element titanium, Ti, can be...
3.2 Periodic trends 1. (0620-5 2012-Paper 1/2-Q21) Which properties of the element titanium, Ti, can be predicted from its position in the Periodic Table? forms coloured compounds conducts electricity when solid can be used has low density as a catalyst X A X X Xx 2. (0620-W 2012-Paper 1/1-Q20) The diagram shows an outline of the Periodic Table. U V W X Y Which of the elements U, V, W, X and Y would react together in the ratio of...
1.) Describe the goals of a Gel Filtration Chromotography
Experiment???
2.) Explain each key theoretical principle of a Gel Filtration
Chromotography, and how they help acheive the goal???.
4.) Explain the key equations used in the Gel Filtration
Chromotography experiment and the terms involved in the
equation????
HI im trying to prepare for a lab/report and i have some
questions i could use help with please :) over all having trouble
seeing how everything ties together etc :) thank you...
Problem 3 (25 p) A 120-V, 60-Hz, three-phase, Y-connected, 6 poles, wound-rotor, synchronous motor has a synchronous reactance of 3 ohms. The motor takes a current of 10 A at 0.8 pf leading when operating at a certain field current. The load is gradually increased until the motor develops its maximum torque while the field current is held constant. Determine: a) The new line current b) The power factor c) Torque developed by the motor
12. A slab on grade measures 120 long x 60" wide x 2' 6" thick. What is the cost to form the slab if formwork costs S1 per square foot of contact area? A. $72 B.S90 C.S162 D.S180 13. A place concrete' activity requires the placement of 2,080 cubic yards. If the individual production rate is 2 cubic yards per workhour per person, with a 10-person crew and 8-hour workday, how many days are needed to complete this activity? A....
180 140 100 200 160 120 80 60 40 20 11 10 9 6 pm 7 3 2 ppm 100- 80- 60- 40- 20- 75 100 125 150 25 50 m/z Relative Intensity o Write all of your notes on the spectra Type a justification of each structure in the same order they appear in your stapled packet o Insert page breaks so each structure is on a separate page o Include page numbers o Type your name in the...
QUESTION:
DATA FOR QUESTION:
60 b. You have determined that ABC would be a more effective costing method. You have decided to 61 split the costs into 3 activities: Routine trip, maintenance, and machine lease-space rent-utilities. 62 Develop an analysis to determine the activity rates for each of the 3 activities 63 Round answers to the nearest cent. Routine Trips Machine lease, space rent, and utilities Maintenance Supervisor Salary Head cashier Salary Associate cashiers Salary ($1,800 each Assoc. cashier) Maintenance...
En (2 points) You isolated your mitochondrial DNA in Part I. In step 6, you discard the supernatant, but keep the pellet. In step 15, you discard the pellet, but keep the supernatant. Explain why the pattern is different between the two steps and the consequence of mixing up these two steps. Procedure Part 1: mt DNA Isolation from your cheek cells. Lysis solution is used to breakdown the cells in this step, you will isolate MEONA from cheek cells....
1. Which of the following is not considered a nutrient a Vitamin B. Water c.Carbohydrate (d) Alcohol e Mineral 2. For which of the following causes of death does a person's diet play a part? a. Lung disease (6) Cancer Chronic lower respiratory diseases d. Infections of the blood e AIDS 3. Researchers repeatedly report that people who consume a variety of foods such as fruits, vegetables, legumes, nuts, and whole grains have reduced risks of which of the following...
3.2 Periodic trends 1. (0620-5 2012-Paper 1/2-Q21) Which properties of the element titanium, Ti, can be predicted from its position in the Periodic Table? forms coloured compounds conducts electricity when solid can be used has low density as a catalyst X A X X Xx 2. (0620-W 2012-Paper 1/1-Q20) The diagram shows an outline of the Periodic Table. U V W X Y Which of the elements U, V, W, X and Y would react together in the ratio of...
Most questions answered within 3 hours.
-
Where is the error in this code sequence?
String s1 = "Hello";
String s2 = "ello";...
asked 10 months ago -
Financial data for Joel de Paris, Inc., for last year
follow:
Joel de Paris, Inc.
Balance...
asked 10 months ago -
Consider this reaction:
Al2(SO4)3 (aq)+ BaCl3
(aq) Al2Cl6 (aq)- +
3BaSO4(s) . What is the...
asked 10 months ago -
Suppose that Savneet is considering increasing her
recent random sample from 20 car rentals to 40...
asked 10 months ago -
Trucks arrive at an unloading terminal at an average rate of 120
per hour.
Trucks arrive...
asked 10 months ago -
Why are methanol and ethanol completely soluble in water while
octanol is not very little soluble....
asked 10 months ago -
A facilities manager at a university reads in a research report
that the mean amount of...
asked 10 months ago -
When the CuSO4 is rehydrated by adding water to the anhydrous
compound, is this an endothermic...
asked 10 months ago -
A ray of sunlight is passing from diamond into crown glass; the
angle of incidence is...
asked 10 months ago -
A block of mass 0.249 kg is placed on top of a light, vertical
spring of...
asked 10 months ago -
how do the kidneys compensate in the presences of acidosis
a) trigger hyperventilate
b) reserve acid...
asked 10 months ago -
Question 501 pts
The rental rate of capital to the firm increases. Which of the
following...
asked 10 months ago