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The following chart shows the data for the oxidation of a substrate to enzyme. The reaction...

The following chart shows the data for the oxidation of a substrate to enzyme. The reaction is followed by monitoring the change in absorbance at 540nm. Create a Michaelis-Menten plot and a Lineweaver-Burk plot. Determine from each plot the KM and Vmax.

[S] (mM) 0.3 0.6 1.2 4.8
Rate (ΔAbs/min) 0.020 0.035 0.048 0.081
0 0
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