How does increasing the polarity of the solvent system affect the results of a TLC?
How does increasing the polarity of the solvent system affect the results of a TLC?
Homework Answers
Solution:
Solvent polarity in TLC playing the important role in the elution. Increasing the polarity of solvents causes a decrease in the Rf value due to higher binding with stationary phase. Since, stationary phase in TLC is highly polar (SiO2,Al2O3), hence increase in polarity increases the attraction with stationary phase, hence it causes a decrease in elution, therefore Rf value decreases.
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How does increasing the polarity of the solvent system affect
the results of a TLC?
If acetaminophen is used in a TLC experiment with 100 % ethyl acetate as the solvent...
If acetaminophen is used in a TLC experiment with 100 % ethyl acetate as the solvent system, explain in terms of polarity why the spot would move fast or slow up the TLC plate?
How does increasing or decreasing the word length parameter affect the run time and results for...
How does increasing or decreasing the word length parameter affect the run time and results for BLAST?
Sometimes elution of a TLC plate with one solvent does not achieve a good separation. How...
Sometimes elution of a TLC plate with one solvent does not achieve a good separation. How could you elute the same TLC plate with two different solvents?
1) What will be the appearance of a TLC plate if a solvent of too low...
1) What will be the appearance of a TLC plate if a solvent of too low polarity is used for the development? too high polarity?
3. How does the shape of a molecule affect its polarity?
3. How does the shape of a molecule affect its polarity?
Does the number of resonance structures affect the polarity of a molecule? How?
Does the number of resonance structures affect the polarity of a molecule? How?
3. If the TLC plate development had stopped before the solvent front reached the top of...
3. If the TLC plate development had stopped before the solvent front reached the top of the TLC plate (i.e. maybe it reached the middle of the TLC plate when it was stopped, instead): a) Would the Rf values have been affected? If so, how? If not, why not? Give a numerical example to support your answer. b) Would the size of the spots have been affected? If so, how? If not, why not? (Hint: think about the diffusion of...
Does TLC depend on solute particle size? I know that it depends on polarity i.e more...
Does TLC depend on solute particle size? I know that it depends on polarity i.e more polar solutes tend to not travel up the TLC plate as far as nonpolar solutes because the silica gel is polar, but does the particle size play any role in it? Also, what is meant by TLC having "pores" in it?
1. When separating mixtures using TLC, why is it important to choose a solvent (mobile phase)...
1. When separating mixtures using TLC, why is it important to choose a solvent (mobile phase) that has a different polarity than the TLC plate (stationary phase)? 2. Why will some substances in a mixture travel farther up the TLC plate than others?
4. TLC Data: Provide a drawing of your TLC plate with proper labels, and specify which solvent system was used to d...
4. TLC Data: Provide a drawing of your TLC plate with proper labels, and specify which solvent system was used to develop the TLC. Comment on the purity of your product according to TLC. Explain why the newly produced phenacetin would run higher on the TLC plate compared to the starting material acetaminophen.
3. How does the shape of a molecule affect its polarity?
1. When separating mixtures using TLC, why is it important to choose a solvent (mobile phase) that has a different polarity than the TLC plate (stationary phase)? 2. Why will some substances in a mixture travel farther up the TLC plate than others?
4. TLC Data: Provide a drawing of your TLC plate with proper labels, and specify which solvent system was used to develop the TLC. Comment on the purity of your product according to TLC. Explain why the newly produced phenacetin would run higher on the TLC plate compared to the starting material acetaminophen.
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