Tris, (HOCH2)3CNH2 (pKb = 5.91), a very common buffer for studying biochemical processes, is prepared by...
Tris, (HOCH2)3CNH2 (pKb = 5.91), a very common buffer for studying biochemical processes, is prepared by a biological chemist using 10.0M NaOH to a pH of 7.79 using 31.52 g of (HOCH2)3CNH3Cl, or TrisH+, (MW=157.597g/mol, pKa 8.075).
(a) Calculate the ratio of [Tris]/[TrisH+] at a pH of 7.79.
(b) Calculate the initial moles of (HOCH2)3CNH3Cl (MW=157.597g/mol) that were added.
(c) Write the chemical reaction between the TrisH+ and the added NaOH.
(d) Calculate the moles of hydroxide used to produce the ratio calculated in part (a).
(e) What volume of 10.0 M NaOH is needed to prepare the buffer?
Now you dilute the solution from above to 1.0 L and take half of the buffer (500.0 mL) and add 0.0100 moles solid HCl. What is the new pH?
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Tris, (HOCH2)3CNH2
(pKb = 5.91), a very common buffer for studying
biochemical processes, is prepared by...
21.) Tris, (HOCH2)3CNH2 (pKb = 5.91), a very common buffer for studying biochemical processes, is prepared...
21.) Tris, (HOCH2)3CNH2 (pKb = 5.91), a very common buffer for studying biochemical processes, is prepared by a biological chemist using 10.0M NaOH to a pH of 7.79 using 31.52 g of (HOCH2)3CNH3Cl, or TrisH+, (MW=157.597g/mol, pKa 8.075). >>>> Need help with second part of question pls>>>>...Now you dilute the solution from problem 21 to 1.0 L and take half of the buffer (500.0 mL) and add 0.0100 moles solid HCl. What is the new pH?
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