Using a fluorescence microscope, you observed how the localization of GFP-Ras changes in response to the...
Using a fluorescence microscope, you observed how the localization of GFP-Ras changes in response to the inhibitor. (8 pts total)
a. GFP is a reporter protein, what does this mean?
b. You used stains to visualize the nucleus and golgi bodies and the fluorescence microscope to determine whether the GFP-Ras protein was present in the golgi or nucleus. How do these stains work?
- i. Hoechst 34580
- ii. BODIPY TR Ceramide
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