Question

Why does the Lineweaver-Burk plot give a more accurate Vmax and Km value compared to a Michaelis-Menten plot?

Answer 1

Lineweaver-Burke plot, the most favoured plot by researchers, has two distinct advantages over the Michaelis-Menten plot, in that it gives a more accurate estimate of Vmax and more accurate information about inhibition.When used for determining the type of enzyme inhibition, the Lineweaver–Burk plot can distinguish competitive, non-competitive and uncompetitive inhibitors.

It increases the precision by linearizing the data. The values for Km and Vmax can thus be determined precisely and the error can be estimated quantitatively.