Explain how to use an SEC (size exclusion chromatography, also known as GPC, gel permeation chromatography)...
In cohesive sentences explain how Size Exclusion Chromatography (SEC) works.
1. In your final year group research work, you did a gel permeation chromatography (GPC) and Differential Scanning Calorimetric (DSC) analysis of two freshly synthesized Polyethylene terephthalate (PET) polymers, labeled as PET1 and PET2. Use the information given in Table to answer the following questions; Table 1. GPC analysis of two synthesized PET PET samples Density(g/cm) PET1 2.122 PET2 2.222 Percentage crystalline (%) 40 70 a. Determine the following parameters; i. Density of completely amorphous phase ii. Density of completely...
Q3. (a) Explain the principle of Size Exclusion Chromatography (SEC). (b) Two polymers of molar mass 21,000 are injected simultaneously in an SEC system. One has Mark-Houwink-Sakurada parameters of K = 2.1 × 10–5 dL.g–1 and α = 0.672, while the other has values of K = 11.4 × 10–5 dL.g–1 and α = 0.716. (i) Will they elute at the same time? If not, which polymer will elute first? (ii) If elution time is proportional to the logarithm of...
1.) Describe the goals of a Gel Filtration Chromotography
Experiment???
2.) Explain each key theoretical principle of a Gel Filtration
Chromotography, and how they help acheive the goal???.
4.) Explain the key equations used in the Gel Filtration
Chromotography experiment and the terms involved in the
equation????
HI im trying to prepare for a lab/report and i have some
questions i could use help with please :) over all having trouble
seeing how everything ties together etc :) thank you...