since v is 20% of Vmax assume Vmax=1, therefore v= 20/100 * 1 = 0.20
We have the relation
1/v = Km/Vmax x 1/[S] +
1/Vmax
1/0.20 = Km/1 x 1/[S] + 1/1
5 - 1 = Km/[S]
4 = Km/[S]
1/4 = 0.25 = [S] / Km
The velocity of an enzyme is 20% Vmax. What is the ratio of [S] to KM...
b. For an enzyme that displays Michaelis-Menten kinetics, what is the initial velocity as a function of Vmax when: a. [S] Km b. S] 0.1 Km c. [S] 50Km c. What will be the initial velocity (yo) for an enzyme that has Km 2.5 [S]? Your answer will be a fraction of Vmax a.
Km = {(Vmax([S]))/(V)} – [S] • Vmax = 100 (units) • At [S] = 20 M • Velocity = 25 (units) calculate Km
An enzyme has a Km of 64.9 mM, determine the fraction (ratio) of Vmax, I.e., v/Vmax, that would be obtained at each substrate concentration below. a. 3.25 mM b. 64.9 mM c. 694 mM d. 6.49 M e. 64.9 M
An enzyme has a KM of 20 µM and a Vmax of 50 mmoles of product/minute/µg of enzyme. After exposure to an inhibitor and analysis on a Lineweaver - Burk plot the following values are obtained: -1/KM = - 0.05 liters/µmole and 1/Vmax = 0.04 (mmoles of product/minute/µg of enzyme)-1. What kind of inhibitor was used in the experiment?
An enzyme catalyzes a reaction with a Km of 9.50 and a Vmax of
1.75
An enzyme catalyzes a reaction with a K_m of 9.50 mM and a of 1.75 mM- s^-1. Calculate the reaction velocity, V_0, for the following substrate concentrations. 2.50 mM 9.50 mM 12.0 mM
Question2 An enzyme solution has a Vmax of 10 μΜ/sec and a Km of 10 μΜ. What is the velocity Vo of the enzyme at the following substrate concentrations? 1 uM 10 uM 100 HM Question3 For an enzyme, the following measurements have been made: Substrate concentration [S] Initial velocity Vo 10 20 40 90 120 180 300 500 10,000 50,000 0.12 0.20 0.30 0.42 0.45 0.39 0.53 0.56 0.60 0.60
What is the velocity of a Michaelis-Menten enzyme reaction (in terms of vmax) when the concentration of substrate is 4 times the value of KM? Show your work.
An enzyme catalyzes a reaction with a Km of 5.00 mM and a Vmax of 1.85 mM s-1. Calculate the reaction velocity, vo. for the following substrate concentrations. a) 3.00 mM Number mM.s b) 5.00 mM Number mM.s c) 10.5 mM Number
An enzyme catalyzes a reaction with a Km of 8.00 mM and a Vmax of 4.45 mM s-1. Calculate the reaction velocity, Vo, for the following substrate concentrations a) 1.00 mM Number mM.s-1 b) 8.00 mM Number mM s-1 c) 11.0 mM Number mM.s-1
What is the rate of an enzyme catalyzed reaction if the Vmax is 100µmol S→P/min and the Km is 7 mM and the substrate concentration is 11mM? Is the enzyme working at Vmax? What if the substrate concentration is raised to 25mM?