A CXR stock solution was diluted 1:2 with saline to make a temporary CXR working solution. The temporary CXR solution was diluted 1:5 with water to make a working CXR solution. 1 ml of the CXR working solution was added to 9 mls of reagent. What is the final dilution of the CXR solution?
A CXR stock solution was diluted 1:2 with saline to make a temporary CXR working solution....
1. You have been asked to make a 1:5 dilution of 20mL of patient sample. The final volume should be 100mL. Which of the following outlines the correct way to make this dilution? For the other answer options, explain why they are incorrect. A. 10mL of patient sample added to 40mL of saline B. 10mL of patient sample added to 50mL of saline C. 20mL of patient sample added to 100mL of saline D. 20mL of patient sample added to...
1) How would you prepare 500mL of working TBE buffer if the stock solution was purchased as 25X TBE? 2) 2mL of a serum sample was added to 18mL of phosphate buffered saline (PBS) in Tube 1. 10mL of Tube 1 was added to 40mL of PBS in Tube What is the dilution of serum in Tube 2? 3) A student needed to prepare 500mL of 1X TAE buffer to run a QC gel. The stock solution in the lab...
1.
2.
Using the equation C1V1 = C2V2, calculate the volume (V1) of concentrated solution and the volume of water needed to dilute the following solutions: Concentration Volume (in mls) Concentration of Volume (in mls) of Volume (in mls) of diluted of desired available stock stock solution required of solvent solution desired solution (V2) solution (C1) to make the diluted required to (C2) solution (V1) achieve desired volume (V2-V1) 5 mg/ml 100 ml 1000 mg/ml 15% 10 ml 50% 0.250...
Make 100 mls of a 10X saline stock solution-- 100mls of 9% sodium chloride. (Please show calculations)
8. A 50-g/L solution was diluted 1:5. This diluted sam ple was then diluted 1:10. What is the concentration of the final solution? a. 0.8 g/L b. 1 g/L c. 2.9 g/L d. 3.3 g/L 9. How much diluent is needed to prepare 300 mL of a 0.2-M working solution from a 0.8-M stock solution? a. 75 mL b. 225 ml c. 900 mL d. 1200 mL 10. What is the molarity of a solution containing 2 mol of sodium...
A working solution is 1× and therefore a 5× solution must be diluted 5 fold, usually with deionised water. Calculate what volume of stock solution is required to prepare 5 litre of 1× TBE buffer from a 100× TBE stock solution. Select the relevant unit. ml or M Calculate the volume of stock solution required.
29. You have the following concentrated commercial reagents on hand; make 1 L of a 6 N reagent from each of them. What is the molarity of each concentrated reagent? How to make it? NFLOH sp. gr. = 0.90; 58% assay (V=1) H(CH3C00) sp. gr. = 1.05; 99% assay (v-1) 30. How much 0.02 N HSO, can you make from 200 mL of 4N HSO.? 31. How much Cuso, 8H20 will you need to weigh out to substitute for 120...
17. One milliliter of plasma is diluted 1/10 in tube 1. Then 1 mL of the dilution in tube 1 is added to 4 mL in a second tube. One milliliter from tube 2 is then added to 9 mL diluent in tube 3. What is the substance (plasma) volume in the third tube? 18. What is the specific gravity of a substance if 5 mL of the substance weighs 6.5 g, but the substance is only 50% pure? 19.What...
Serial Dilution Questions
1, Serial dilution Questions: a) (5 pts) Suppose you begin with stock solution of dye that has a concentration of 350.0 mg/L and do the following set of serial dilutions on it: 5.00 mL of stock diluted to a total volume (TV) of 500.00 mL (Dilution 1); 2,00 mL of Dilution 1 is then diluted to a TV of 250.00 mL (Dilution 2); finally, 1.00 mL of Dilution 2 is then diluted to a total volume of...
1. Serial dilution Questions: a) (5 pts) Suppose you begin with stock solution of dye that has a concentration of 350.0 mg/L and do the following set of serial dilutions on it: 5.00 mL of stock diluted to a total volume (TV) of 500.00 mL (Dilution 1); 2.00 mL of Dilution 1 is then diluted to a TV of 250.00 mL (Dilution 2); finally, 1.00 mL of Dilution 2 is then diluted to a total volume of 100.00 mL. Calculate...