Calculate the amount of agarose and 10,000x SYBR safe needed to make 15 mL of 1%...

Question

Question

Calculate the amount of agarose and 10,000x SYBR safe needed to make 15 mL of 1% agarose 1x SYBR safe

science chemistry

Answer 1

Answer #1

1% agarose = 1g/100mL solution .

Thus for 15mL solution we need = (1g/100mL)×15mL = 0.15g of agarose powder.

For SYBR safe :

C1V1 = C1V2

10000X × V1 = 1X × 15mL

V1 = (1×15)/10000 = 0.0015 mL

Thus we require, 0.0015 mL of 10000X SYBR safe

And 0.15g of agarose powder for the required TAE .

Answer 2

Similar Homework Help Questions

1. How would you calculate the amount of agarose powder you would need to make 35mL...

1. How would you calculate the amount of agarose powder you would need to make 35mL of a 2% (wt/vol) solution? 2. How would you calculate the volume of 20X concentrated buffer to make 35 mL of a 1X solution?
You need to prepare a 0.5% (w/v) agarose gel containing 1X TAE and 1X Gel Red....

You need to prepare a 0.5% (w/v) agarose gel containing 1X TAE and 1X Gel Red. For a total gel volume of 75ml, calculate the mass of agarose powder and volume of Gel Red concentrate needed. Show your work to be eligible for partial credit. Info about stocks: Agarose stock is powder Gel Red concentrate is 25,000X liquid
Petermine the amounts/volumes of the chemicals needed to prepare the following solutions s 100 ml...

9,10,12 petermine the amounts/volumes of the chemicals needed to prepare the following solutions s 100 ml of O.1 N HC from concentrated (12.0 M) HO tyou will be asked to make this solution as practicel & 100 ml of L.0 M Tris pt-76 MW of Tris base is 121 g/mole 7. pH 7.6 is close to a neutral pH. When making 1.0 M Tris base, would you expect to or NaOH to adjust the pH to 7.67 to need HC...

To do electrophoresis, you will need to make a solution called TAE, which is a specific...

To do electrophoresis, you will need to make a solution called TAE, which is a specific mixture of Tris base, acetic acid, and EDTA. Assuming no errors and no spills, you will need a minimum of 50 ml 1X TAE to make your agarose gel plus 1 liter of 1X TAE for gel running buffer. TAE is normally made as a 50X concentrated stock.
Gel Electrophoresis and Bioinformatics Analyses of wild type and mutant Arabidopsis 1. The procedure for making...

Gel Electrophoresis and Bioinformatics Analyses of wild type and mutant Arabidopsis 1. The procedure for making 30 mL of 1.2% agarose in 1x TAE is: g agarose mL lX TAE 2. Briefly explain the roles of each of the following in electrophoresis. Agarose - Tris - EDTA- Power supply - 3. What is the stain we used to view DNA and how does it work? 4. Which samples amplified and which ones did not?
1) Calculate the amount of NiSO4·6H2O needed to make a 10.0 mL of 0.100 M NiSO4....

1) Calculate the amount of NiSO4·6H2O needed to make a 10.0 mL of 0.100 M NiSO4. 2) Calculate how many mL of concentrated ammonia (15.0 M) to make a 20.0 mL of a 5 M solution 3) What does the term reflux mean (in context of chemical synthesis)?

Can anyone show me step-by-step on how to do the agarose calculations? This week we will...

Can anyone show me step-by-step on how to do the agarose calculations? This week we will run the PCR reactions on an agarose gel and analyze the results. Safety notes: Ethidium bromide is a potent mutagen. Wear gloves when handling containers containing ethidium bromide, when handling gels that have been stained in ethidium bromide, and when working with the computer attached to the gel-doc system. Protocol I. Pouring agarose gels I. Using 10x TAE, prepare 50 ml of 3% (w/v)...
1- Calculate the number of grams needed to make 1 mL of a 1M solution of...

1- Calculate the number of grams needed to make 1 mL of a 1M solution of the oligonucleotide (32nt) 2- You have a 300 μM solution of a primer (45nt) in a volume of 400μL, how many μg of primer do you have total? 3- You received a primer (25nt) which came to you as a powder with a mass of 0.31 mg a- what concentration of primer do you have when you add 100μL water? b- How would you...
help asap! easy! bio! If you wanted to make 60 ml of a 1% agarose gol...

help asap! easy! bio! If you wanted to make 60 ml of a 1% agarose gol in TBE, how many grams of agarose do you need? What is the TBE used for in the gol and in the gol box? T TTT Paragraph : Arial 3(12pt) .E. T. %DO Q E T' T. - -

Calculate the amount of Iron standard needed to make 1L of 15 ppm iron (Fe2+), from...

Calculate the amount of Iron standard needed to make 1L of 15 ppm iron (Fe2+), from ferrous ammonium sulfate hexahydrate