Question

Substrate concentration (µM) Reaction rate (µmole/min) 0.08 0.15 0.12 0.21 0.54 0.7 1.23 1.1 1.82 1.3...

Substrate concentration (µM)

Reaction rate (µmole/min)

0.08

0.15

0.12

0.21

0.54

0.7

1.23

1.1

1.82

1.3

2.72

1.5

4.94

1.7

10

1.8

Construct a lineweaver graph using the data above. Please show how the Km and Vmax were determined.

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Answer #1

I plot the graph of 1/V Vs 1/[S]. From the we get y intercept and slope to calculate the Vmax and Km.

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