|
Substrate concentration (µM) |
Reaction rate (µmole/min) |
|
0.08 |
0.15 |
|
0.12 |
0.21 |
|
0.54 |
0.7 |
|
1.23 |
1.1 |
|
1.82 |
1.3 |
|
2.72 |
1.5 |
|
4.94 |
1.7 |
|
10 |
1.8 |
Construct a lineweaver graph using the data above. Please show how the Km and Vmax were determined.
I plot the graph of 1/V Vs
1/[S]. From the we get y intercept and slope to calculate the Vmax
and Km.


Substrate concentration (µM) Reaction rate (µmole/min) 0.08 0.15 0.12 0.21 0.54 0.7 1.23 1.1 1.82 1.3...
Name Page Number Date Vmax 1. Estimate Vmax and Km using the velocity vs. substrate concentration plot - KM 2. Calculate the Vmax and Km using the Lineweaver-Burk plot. KM Vmax (S), uM 0.08 0.12 0.54 1.23 1.82 2.72 Reaction Velocity (UM/min) 0.15 0.21 0.70 1.1 1.3 15 4.94 10.00 1.8
(15 points) The following data is for a reaction
catalyzed by tyrosine monoxygenase:
Substrate
Concentration
(mol/L)
Initial Velocity (mM/min)
1.5
0.66
1.2
0.65
0.81
0.45
0.65
0.39
0.49
0.32
0.27
0.21
a) Plot the velocity (y-axis) versus substrate
concentration [S] (x-axis) curve and insert/draw the graph in the
space below. What are the approximate KM and
Vmax values?
b) Construct a 1/v (y-axis) versus 1/[S]
(x-axis) plot in the space below. Calculate the KM and
Vmax values.
c) Calculate the...