Calculate the weight of the following buffers: CAPS, Acetate, Citrate, and HEPES, that is needed to...

Question

Question

Calculate the weight of the following buffers: CAPS, Acetate, Citrate, and HEPES, that is needed to make 100ml of a 0.1M buffer.

science chemistry

Answer 1

Answer #1

The weight of each salt required to prepare the buffer is calculated:

m CAPS = M * V * MM = 0.1 M * 0.1 L * 221.32 g / mol = 2.21 g

m Sodium Acetate = 0.1 * 0.1 * 82 = 0.82 g

m Sodium Citrate = 0.1 * 0.1 * 258 = 2.58 g

m HEPES = 0.1 * 0.1 * 238 = 2.38 g

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Answer 2

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