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why is the max peak used so that beer’s law is obeyed in the uv-vis spectra?...

why is the max peak used so that beer’s law is obeyed in the uv-vis spectra? explain using equations
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Answer #1

According to equation, A (absorbance) = ecl,

Plot is being drawn between absorbance and concentration.

When absorbance is high, concentration is also high. When concentration is low, signal to noice ratio decrease as signal is very low therefore noice increase. Hence, chances of error also increase. Whereas, when concentration is high, signal to noice ratio Decrease as signal increase noice decrease. Due to decrement, chance of error also decrease. Therefore, in order to do accurate calculation, we use max peak for UV- Visible spectra

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