The following data were obtained. Which enzyme has a higher affinity for substrate? (Assume k2 the...
The following data were obtained. Which enzyme has a higher affinity for substrate? (Assume k2 the rate-limiting step) What is the initial velocity of the kinase reaction at a substrate concentration of 10 uM? Suppose the amount of input enzymes used in this study is 10 nM. What is the kcat and specificity constant for wild type and mutant enzymes? What conclusion you can reach about the mutant enzyme? Which enzyme shifts Keq, the equilibrium constant, more in the direction of product?
|
Maximum Velocity |
Km |
|
|
WT Ste7 |
100 nmol/min |
10 uM |
|
Mutant Ste7 |
1 nmol/min |
0.1 uM |
Homework Answers
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The following data were obtained. Which enzyme has a higher
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For her undergraduate project, Jessica studied an enzyme that catalyzes the reaction A B. For substrate A, she determined 30 min that Km 3.0 HM and kcat Jessica graduated and her project has been passed on to you. Unfortunately, Jessica was so busy that she sometimes forgot to record all of the details of an assay in her lab notebook. Your mentor suggests that you try to back calculate some of the missing concentration values. Assume that the enzyme follows...
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