Question

The efficiency of that energy transfer, given by ??, has a distance dependence described by the equation ?? = ?0 6 ?0 6+?6 . FRET can be used at the molecular level to determine the distances between different parts of molecules/proteins. In plant photosynthesis the absorption of light is done chlorophyll molecules, where the fluorescence from the absorbing chlorophyll molecule is quenched by nearby chlorophyll molecules attached to a reaction center. Given that for a pair of chlorophyll molecules R0 = 5.6 nm, by what distance should two chlorophyll molecules be separated to shorten the fluorescence lifetime from its normal lifetime of 1 ns to 10 ps?

Answer 1