Question

You have 2 test tubes, one tube contains neutrophils that only express TLR receptor, another tube contains macrophages and only express CR1 receptor. However, these two tubes are not labeled, so you need to figure out the identity of these two tubes.

1) what is the ligand for TLR?

2) what is the ligand for CR1?

3) To determine which tube will contain neutrophils that expressing TLR only, a sample will be taken from both tubes. To each of those samples, I will add ______ samples? Then I will measure __________in both samples?  

4) The ligand for CR1 functions as a/an _____? Thus, to determine which tube contains macrophages expressing CR1 only, I will take the sample from both tubes, and to each of those samples I will add ____+ _______? Then I will measure ______ (must be different from question 3) in both samples.

5) Demonstrate the expected results if text tube "#1" contains mast cells with TLR and test tube "#2" contains macrophages with CR1. Labels y-axis and every bars. Each graph should have 4 different groups represented by 4 different bars. The first graph is for question 3, another graph is for question 4.

6) explain your answers for each question.

Answer 1

Answer 1:)

Ligands for TLRs are bacterial lipoprotein, zymosan cell wall particles, synthetic dsRNA, lipopolysaccharide, and flagellin.

Answer 2:)

The ligands for complement receptor type 1 (CTR1) are C3b, C4b, C1b, and iC3b.

Answer 3:) TLR ligands; OD₆₀₀

Add ligand for TLRs in both test tubes and then measure OD₆₀₀ of both samples.

Answer 4:) conjugatory; TLR ligands + CR1 ligands; coagulation

Ligands of CR1 are multimeric proteins and conjugates with receptors. Therefore, use both ligands: TLR and CR1. Then measure ELISA interaction of both samples by coagulation analysis.