HomeworkLib
Question

You need to prepare 100.0mL of a 50.0mM phosphate buffer with a pH of 7.00. The...

You need to prepare 100.0mL of a 50.0mM phosphate buffer with a pH of 7.00. The pKa for this form of phosphate is 6.82. Calculate the weight of NaH2PO4*H2O and volume of 1.00M NaOH that you will require.

science   chemistry   
0 0
Add a comment Improve this question Transcribed image text
Next > < Previous
Sort answers by oldest

Homework Answers

Answer #1

0 0
Add a comment
Know the answer?
Add Answer to:
You need to prepare 100.0mL of a 50.0mM phosphate buffer with a pH of 7.00. The...
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Log In

Sign Up

Not the answer you're looking for? Ask your own homework help question. Our experts will answer your question WITHIN MINUTES for Free.
Similar Homework Help Questions

  • Preparation of Phosphate Buffer Rxn: Purpose: The purpose of lab this week is to prepare a...

    Preparation of Phosphate Buffer Rxn: Purpose: The purpose of lab this week is to prepare a 0.05M sodium phosphate buffer, use a pH meter to adjust the pH of this buffer, and to calculate theoretical pH changes upon addition of acid/ base. Your theory will then be correlated against your actual observational pH changes. Solutions to be made Molecular Weight Table Solution Volume 1.0M HCL 10ML 1.0 M NaOH 20ml 0.05M Sodium Phosphate: *?g NaH2PO4 H2O + *?g Na2HPO4 7H2O,...

  • Sample Buffer Calculation: Given a pH of 5.4, prepare a buffer using one of the buffer...

    Sample Buffer Calculation: Given a pH of 5.4, prepare a buffer using one of the buffer systems below: System A: 3.5M acetic Acid (MM=60.1) : sodium acetate, anhydrous (MM=82.0) pKa = 4.74 System B: Sodium phosphate, monobasic (NaH2PO4 x H2O) (MM=138.0) : Sodium phosphate, dibasic, Na2HPO4 (MM=142.0) pKa = 6.86 a) Choose the buffer system most effective at pH of 5.4 b) Calculate the amounts of weak acid and conjugate base you need to make 25mL of 0.25M solutions (one...

  • 1. You have a 1L solution of 0.1 M phosphate buffer at pH 2.0. What is...

    1. You have a 1L solution of 0.1 M phosphate buffer at pH 2.0. What is the pH of this solution after you add 5 grams of NaOH? Assume there is no volume change and phosphate buffer has the following pKa values: 2.15, 6.82 and 12.38.

  • pka=6.86 Calculations. Preparation of 0.1M phosphate buffer pH=74 1. Calculate the amount mL this buffer. O...

    pka=6.86 Calculations. Preparation of 0.1M phosphate buffer pH=74 1. Calculate the amount mL this buffer. O of NaH2PO4 (MW=120g/mol) you will need to prepare m 100 added to prepare 100ml of phosphate buffer 2. Calculate how many ml of 1M NaOH must be at pH=7.4. MOOS. O Imo.ea gnizim vd som slud 1096 MOOS.O ses to Horse Snodulo bios MOOS.Os lo Im 0.0a bris not to muit

  • Calculate the concentration of disodium phosphate (Na2HPO4) and monosodium phosphate (NaH2PO4) to prepare of 100.0mM buffer...

    Calculate the concentration of disodium phosphate (Na2HPO4) and monosodium phosphate (NaH2PO4) to prepare of 100.0mM buffer at pH 7.50. the pKa of H2PO4= 7.21

  • How would you prepare 10L of 0.045M potassium phosphate buffer, pH 7.5? (1 point for correctly...

    How would you prepare 10L of 0.045M potassium phosphate buffer, pH 7.5? (1 point for correctly identifying which phosphate species, and 1 point for providing the needed weight of each to make the buffer) Hint – to do this you will need to calculate a weight for each phosphate species (to either side of the desired pH); you can determine which species will be needed based on their pKa values.

  • Create a buffer solution with a pH of 6.0. Based on the assigned pH, you will...

    Create a buffer solution with a pH of 6.0. Based on the assigned pH, you will choose an appropriate buffer system. pH = pKa + log[A-}/[HA] A) 3.5M acetic acid 60.1MM, sodium acetate, anhydrous 82.0MM, pKa 4.74 OR B) sodium phosphate, monobasic, NaH2PO4*H2O 138.0MM, sodium phosphate, dibasic, Na2HPO4 142.0MM, pKa 6.86 Calculate the amount of weak acid and conjugate base you need to make 25mL of 0.25M solutions (one acid and one base solution).

  • You need to prepare two buffer solutions for biochemistry lab. Describe how you would prepare the...

    You need to prepare two buffer solutions for biochemistry lab. Describe how you would prepare the two buffers below: (1) 1.00L of 0.100 M acetate buffer at pH 4.00. pKa acetate: 4.75 (2) 1.00 L of 0.100 M sodium phosphate buffer at pH 7.00 pKa sodium phosphate: 7.21

  • a) What are the concentrations of Na2HPO4 and NaH2PO4 in a 0.30 M phosphate buffer solution...

    a) What are the concentrations of Na2HPO4 and NaH2PO4 in a 0.30 M phosphate buffer solution pH 7.0? Use pKa 6.82 b) Describe how you would prepare 250 mL of the buffer in part (a) given that you have available to you a 1.0 M stock solution of NaH2PO4 and solid Na2HPO4(FW 141.96 g/mol). Provide your answer in milliliters of NaH2PO4 and grams of Na2HPO4. c) Suppose you use 100 ml of this buffer in an experiment and 0.003 mol...

  • Calculate how you will prepare 0.1M buffer solutions ranging from pH 1-12 in increments of 1...

    Calculate how you will prepare 0.1M buffer solutions ranging from pH 1-12 in increments of 1 pH unit. NaOH and HCl droppers will be available to make adjustments to the final pH. a. pH 9 Tris Base (pKa 8.08, 121.14g/mol) and Tris-HCl (151.59g/mol) are available. b. pH 10, 11, 12. CAPS (3-(Cyclohexylamino)-1-propanesulfonic acid, 221.32g/mol, pKa 10.4) is available. c. pH 7, 8. Dibasic potassium phosphate (pKa 6.82, 174.18g/mol) and monobasic potassium phosphate (136.09g/mol) are available

ADVERTISEMENT
Free Homework Help App
Download From Google Play
Scan Your Homework
to Get Instant Free Answers
Need Online Homework Help?
Ask a Question
Get Answers For Free
Most questions answered within 3 hours.
ADVERTISEMENT
ADVERTISEMENT