If two bands are not fully resolved in a reverse-phase liquid chromatography experiment when using a...
If two bands are not fully resolved in a reverse-phase liquid chromatography experiment when using a mobile phase of 50:50 water: acetonitrile, which of the two components of the mobile phase should you increase the percentage of in order to improve the resolution? Explain how this change will improve the peak separation?
Homework Answers
In a reverse phase chromatography stationary phase is nonpolar and mobile phase is Polar
so as mobile phase moves up it separates the non polar compound from polar. it takes polar molecules with it, thereby achieving a separation.
in this case given in the question to improve the resolution we need to to increase polarity of the solvent. for this the amount of acetonitrile in mobile phase solvent should be increased because acetonitrile is more polar than water.
This will give a better and good resolution. This will result in better movement of Polar molecules thereby giving a good peak separation.
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If two bands are not fully resolved in a reverse-phase
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Should you use a higher or lower concentration of acetonitrile in
the next run?
(b) When you try separating an unknown mixture by normal-phase
chromatography with 50% hexane/50% methyl t-butyl ether the peaks
are too close together and eluted in the range k = 2-6. Should you
use a...
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