(biochem question) What reaction does alkaline phosphatase catalyze
Alkaline phosphatase include a group of isoenzymes present on outer layer of cell membrane. All alkaline phosphatases hydrolyse the organic phosphate esters present in extracellular space.
biochem
In addition to catalyzing the decarboxylation of an a-ketoacid, TPP can catalyze a synthesis reaction using the decarboxylated intermediate. The TPP-dependent enzyme acetolactate synthase catalyzes the coupling of two molecules of pyruvate to generate acetolactate, a precursor to the synthesis of valine and leucine in plants. Propose a TPP- dependent mechanism for this reaction. H3C H3C HOCH, Pyruvate Acetolactate
1.What can we conclude about the expression of alkaline phosphatase in pork and liver tissues?Is alkaline phosphatase expressed in all tissues? In equal amounts? 2.Are there any problems that might make one tissue more difficult to examine than an other? 3.Why do you think the enzyme is expressed in the cells that it is? 4.Is the expression of the enzyme localized in any particular areas of the tissues? Is it all over? How can you tell?
Question 4. Consider the protease chymotrypsin. a. What reaction does it normally catalyze? b. Can it catalyze the formation of peptide bonds? Why or why not? c. Where is chymotrypsin active in the body? Can it catalyze formation of peptide bonds in this location? Why or why not?
1. In a phosphate limitation environment, E. coli will be induced to produce alkaline phosphatase. (A) What is the function of alkaline phosphatase? And how does it help the bacteria to survive in this environment? (B) The phosphate sensing system of E. coli is an example of sensor kinase system. Describe the molecular mechanism of the phosphate sensing system of E. coli using the sensor kinase model. 2. Pseudomonas aeruginosa is used as a bacterial model to study biofilm mechanism....
1. Alkaline phosphatase is an enzyme which a. transfers electrons to orthophosphate, converting it to elemental phosphorous b. adds a phosphate group to adenosine diphosphate c. reacts an organic phosphate compound with water, producing an alcohol and inorganic phospahte 2. In this assay for acid phosphatase activity, we use the spectrophotometer to measure the rate of appearance of a. nitrophenol, which is yellow b. potassium phosphate c. hydroxide ions, raising the pH d. protons, lowing the pH 3. The Triton...
Question 12 How does an enzyme work to catalyze a reaction? -It lowers the activation energy of a reaction. -It raises the temperature of a reaction. -It supplies the energy to speed up a reaction. -It increases the concentration of the reactants in a reaction. -It allows the reaction to proceed through different intermediates.
Use Table 1 and Table 2 to create (1) a graph showing alkaline phosphatase activity at different pH values and (2) a graph showing enzyme activity at different temperatures. Label the x and y axes. Be sure to use appropriate units for specific activity (from answer 3 above). Table 1. Bacterial alkaline phosphatase activity was measured at 70�C and various pH values. pH value Specific activity 7.0 0.060 8.0 0.075 8.5 0.170 9.0 0.270 9.5 0.325 10.0 0.280 Table 2....
45.Patient age 19 years of age presents with the following laboratory test results. The alkaline phosphatase result Reference Interval 2.1-2.62 mmol L 0.8-1.45 mmol L 115 UL 60-85 gL 35.55 @L 166-738 pmol L 475-2220 nmol/L absorption % ESg in 6h Result 1.5 0.65 210 50 28 100 221 4.2 43.59% excreted 3.5g Test Calcium Phosphorus Alkaline phosphatase Total protein Albumin Vitamin B12 Folate, rbc Schilling Fecal fat Xylose test Urine on Albumin Glucose Blood RBC Admission negative negative Begative...
Tell me two uses for carbamoyl phosphate in metabolism. What reaction does rubisco catalyze?
1. One enzyme will generally catalyze: a. Does not catalyze any reaction b. Only one unique reaction c. Several different reactions d. many different reactions e. none of the above 2. List three experimental conditions which would affect enzyme activity: (six different conditions were done in lab) 3. enzyme Reaction: ------> Ris called: Q is called: 4. One experimental method that would destroy enzyme activity: