Question

A malate dehydrogenase kinetic assay uses a 100mM potassium buffer, pH 7.5. Assume that you have available-- phosphoric acid (15M), mono potassium phosphate (MW 136.1), dipotassium phosphate (MW 174.2), tri-potassium phosphate (MW 212.3). Show how you would prepare 100mL of this potassium buffer for your malate dehydrogenase assays.

Answer 1