Question

Preparing samples for H NMR analysis:
Why do I add deuterated chloroform into ethyl acetate before transferring it to a NMR tube?
Similarly, deuterated chloroform is added to vanillin for the second sample.

Answer 1

ANSWER:

We are adding deuterated chloroform or other deuterated solvent in the NMR tube along with our sample because

• To facilitate locking and shimming process
• To suppress the large solvent peak that would otherwise occur in the proton NMR spectra
• To stabilize the magnetic field strength