Question

Question:

In this lab, you are going to lyophilize your protein solution. Why is it important to make sure that the liquid is not frozen into one block of ice before subjecting it to the vacuum?

1. If the solution is in one block of ice, the lyophilizer will not work because the tubes become bottom-heavy.

2. If the solution is in one block of ice, not all of the water will evaporate.

3. If the solution is in one block of ice, the solid that is produced by lyophilization is too fluffy for future use.

4. If the solution is in one block of ice, less surface area of the solution is subject to the vacuum, and thus there is a lower rate of water sublimation.

Answer 1