What does the slope of the absorbance change over time represent in a Lineweaver-Burk plot?
A Double-Reciprocal or Lineweaver-Burk Plot. A double-reciprocal plot of enzyme kinetics is generated by plotting 1/V0 as a function 1/[S]. The slope is the KM/Vmax, the intercept on the vertical axis is 1/Vmax, and the intercept on the horizontal axis.
What does the slope of the absorbance change over time represent in a Lineweaver-Burk plot?