Question

You have just trypsinized your monolayer cells growing in a T25 flask. Once detached you resuspended all of your cells in media. If you were to subculture some of your cells into a new T25 cells by taking 1 mL of cell suspension from a total of 9 mL and transferring it into a new T25 flask containing 5 mL of fresh media, your final dilution would be:

Answer 1

Once detached you resuspended all of your cells in media.

Total , cell suspension : of 9 mL

1 ml of cell suspension from this to a new T25 flask containing 5 mL of fresh media =>

           diluted volume : 1 mL + 5 mL = 6 mL

your final dilution => 1 mL : 6 mL = 1 : 6