Question

Penicillin is hydrolyzed and thereby rendered inactive by beta-lactamase, an enzyme present in some penicillin-resistant bacteria. The mass of this enzyme is 30.0 kDa. The amount of penicillin hydrolyzed in 1 minute in a 1 mL solution containing 3 x 10-3 g of the enzyme was measured as a function of the concentration of penicillin. Calculate Km, Vmax , and kcat from the following data: (show your calculations and your Lineweaber-Burk plot on the graph paper.) (Clearly show where 1/Vmax and -1/Km are; clearly explain how to obtain your kcat value).

[S] (mM)	Vo (mM s^-1)
0.14	0.138
0.28	0.234
0.55	0.333
0.77	0.338
1.52	0.491

Answer 1