Question

Urate oxidase is one of the most interesting enzymes in the world because it carries out a spin-forbidden oxidation of urate with molecular oxygen without any cofactors. Meticulous kinetic studies have shown that the K_m of urate oxidase toward urate is 10 μM while the turnover number of urate oxidase under slightly basic conditions is about 4 sec^-1.

Calculate the concentration of remaining chorismate after 7 hours when 10 mL of 100 μM chorismate solution is mixed with 10 mL of 100 nM solution of urate oxidase at pH 7.2 and the solution is incubated at 37 °C while continuosly stirring in the open container to maintain the oxygen concentration at the constant value of 267 μM

Answer 1

The solution can be found in the following 3 images