Question

please help me with this. label A & B and provide all the details.

6. You developed a three point standard curve (each point in triplicate) for quinine revealing the following fluorescence intensities at a given concentration. ppm 0.1 0.001 0.00001 fluorescence intensity (FU) 690, 762, 725 4.8, 9.5, 5.8 0.16,0.25, 0.21 Conc A) Plot this data including error bars to develop the standard curve. What is the limit of detection based on this data set? You then extracted 10 grams of chichona bark with 50 mM H,SO,, producing 45 ml of extract After filtering and dilution by a factor of 10,000 a fluorescence intensity of 64 was obtained. What is the concentration of quinine in your extract in micrograms per mi? and what is the concentration in the original bark in micrograms per gram? B)

Answer 1

Goo 500 300 200 -(o0 Contertration (pm) 0 13141 48.81o18 Slopc 240-370

7246 -6 armn So, i혼 ·A 0.0089 micro gram 1m) 50 (oplentvarįơn },35/00 00X0008 om 89 micro gramim