Question

To generate the supernatants that you used to measure a_635, you had to centrifuge the samples to remove the solid. If you did not centrifuge the samples properly, what impact would that have on the measured a635 and on the [cu2+] that you would calculate?

Answer 1

Before measuring the absorbance we should centrifuge the samples and remove the solids it is very much important as we are measuring absorbance means how much light the compound is absorbing at particular wavelength so if we do not centrifuge the sample then there are more chances of getting solid particles into the sample and due to which the solution becomes some what turbid or we can say it will become a colloidal solution and due to which it obviously affect the absorbance as well as concentration also solid particles also block the wavelength so that also affects the possible outcomes of the concentration as well as absorbance values.