Question

5. Which of the following light wavelengths is emitted by CMXRos, but not absorbed by it? (a) 650 nm (b) 575 nm (c) 525 nm (d) 450 nm (c) 375 nm lipop ilic-combine/aucier m ipd or fata 1 Page 1110 CMXRos (chloromethyl-X-rosamine)-Scins mitochorriria by roudina wlcthio CMXRos is a lipophilic cationic molecule (Figure 8.2) that live eukaryotic cell and accumulates in the mitochondrion. The molecule has a chloromethyl group that reacts with thiol groups on proteins of the outer and inner mitochondrial membranes, resulting in the covalent attachment of CMXRos to said proteins, CMXRos is also capable of fluorescence. Its excitation and emission spectra are presented in Figure 8.3 below, rough the plasma membrane of a Excitation spec 57 nm CH,CI Figure 8.2. Molecular structure of CMXRos 100 EXC: 579 nm EMI: 599 nm 75 50 25 600 Wavelength (rm https:/www.thermofisher.com/order/catalog/product/ M7513 Figure 8.3. Excitation /emission spectra of CMXRos.

Answer 1

The excitation spectrum of CMXR_OS has the maximum at 579 nm while the emission spectrum has the maximum at 599 nm. At 575 and 525 nm wavelengths, CMXR_OS shows appreciable absorption; however, there is no emission at 525 nm. Similarly, at 375 nm, CMXR_OS has only an absorption peak and no emission. At 450 nm, CMXR_OS has no absorption or emission peak. Only at 650 nm, CMXR_OSshows an emission peak, but there is no absorption of light at this wavelength. Therefore, the correct answer is (a) 650 nm.