Question

accordingly answer and i pt): Polymers and Monomers. Read each question chemical structure, in a oymer chemical structure based on the polymer name and provide the monomer unit chemical structure, in addition. Lastly applications. An example answer is provided as guidance. , provide an example of how the polymers are utilized in (bio)engineering Polymer Chemical Structure Polymer Full Name and Abbreviation Usage Example for Monomer Unit Structure(s) _ _pt per box) pt per box) pt per box) Nucleic Acid (DNA) Gene Delivery Base Polystyrene (PS) Polylactic acid (PLA) Polycaprolactone (PCL) Polylysine (PL) Sodium alginate (Na-Alg) Question 2 pt): Cell Counting and Passaging provide a step-by-step procedure for passaging and media changing C6 cells. (2A, 96) Please Answer for 2A: (2A,--96) Please calculate the total number of cells you have. The total volume for your cell stock suspension is 2 ml. No dilution was performed. The numbers below represent five 100 nL volumes you counted. Basically, you directly measured the cells in 100 nL increments (five times) from the 2 mL stock suspension using a hemocytometer. Show your work. You counted five spaces (0.1 μL volumes) in a regular hemocytometer and got the following numbers: 170, 169, 151, 162, 148. Answer for 2B: uestion 3 pt): Categories of Biomaterials. example materials to their materials categories listed in the blue table below. The method of physicochemical & biological properties (and their ultimate application). pt) Match the categorization may be based on the materials Please choose one per box. Example Materials G. Electrospun Collagen Fibers (mm in length, nm in diameter) H. Titanium Alloy Hip or Joint Replacement I. Calcium Chloride (dissolved) in Water J. Poly-styrene Extended Surface (e.g. T25 Flask) K. Dye molecule (e.g. Oil-Red-O, methylene blue) L. Fetal Bovine Serum (Proteins) A. Sodium Alginate (for Hydrogels) B. Poly-L-Lysine Macromolecules (Polymers) C. Lipid Conjugated Arg-Gly-Asp Peptides D. 100 nm Fluorescent SiO2 Nanoparticles E. Polydimethylsiloxane (PDMS) Membranes F. 500 um (0.5 mm) Poly(lactic acid) Microspheres Materials Categorization Table_pt per box) Materials Category Answer(s) Answer(s) Materials Category Hard Particles 1Naturally Ocourring Polymer 2 Synthetic Polymer 3 Ceramic (Metal Oxide) Soft Particles Fibrous Materials Metal 9 Sub-1 nm Inorganic lons 10 Small (Sub-1 nm) Molecules 5 Hybrid Organic and Inorganic (Q3B, pt) The bioengineering company that you are working for just received a request to produce an injectable drig delivery system (DDS) specific for targeting lung cancer cells to detect and kill them. The requesting organization wants to use a system that can be utilized for human clinical trials later. This means your company and the requesting organization will need to ultimately apply for an FDA (Food and Drug Administration) approval. (Q3Ba) Which example materials would you utlize from 3A for the DDS? Answer (choice) for 038a: (Q3Bb) Please provide a (materials) design example of your drug delivery system here utilizing component illustrations, text labels, and brief materials design explanations. For your design, considering the ollowing tems: (1) Length scales, (2) Functions as a DDS, (C) Biocompatibility, and (4) Bloactivity to achieve the specific aims detailed in the question. Basically bels le of Answer (explanation) for Q3Bb pt): Draw Design Illustration Here Question 4 pt): Biocompatibility Testing t pt) Please explain how you will experimentally conduct blocompatibility testing of your chosen m (DDS) from Q3B (previous question). Provide easy to follow steps including the cellular etails. Assume that you are providing a procedural overview to your newest company employees ded vu charge of training them to test biocompatibility of new materials. Additionally, your company (Q4A, drug delive experimental detail has provided you with multiple tetrazolium-based assay kits Answer (explanation) for Q4A: (Continued from Q4A) (Q4B This question is about the critical chemical reaction stepfs) for the (biochemical assay) kit you blocompatiblity testing detailed in Q4A - the tetrazollum system. pt) the utilized for (Q4Ba, pt) Indicate whether or not the following specific reaction scheme is a reductinor an process. Why did you decide on the particular reaction type? Please provide a short (Q4Bb,-pt) Please write down which molecule is tetrazolium and the other formazan in the table below (Q4Bc pt) Explain what you are actually measuring when you use the tetrazolium system to conduct biocompatibility assessments. Write down what components of a cell will need to be utilized and during what conditions. How is this a quantitative approach? Answer for Q4Ba and Q4Bb: Q4Ba. Reaction Type Oxidation or Reduction (Circle One). Why? Q4Bb. Name (Answer) Chemical Structure Chemical 1 Chemical 2 Answer for Q4Bc: Provide the detailed chemical reaction explanation here for tetrazolium-based biocompatibility testing. Question 5 %pt): Polymers and 3D Structures. Please explain why only presence of calcium ions (Ca2) and the rest will not. Answer the series of questions detailed below Table Q5. The chemical structure of polymers. one of the structures in the Table (Q5) below will produce a 3D hydrogel in the Name Structure(s) Structure(s) Name PLGA A Sodium Alginate Poly(lactide- co-glycolide) PLL D Cellulose Poly-L-iysine (05A.) Frst, draw (in the boxes) the chemical structures of each polymer based on the provided names. (Q5B,pt) Which structure wil form a hydrogel with calcium ions? Answer (choice) for Q5B: (QuC, , iiii tease axplain why you chose the particular answer for Q5B. When formulating the answer, please provide a pictorial (illustrative) view of the internal crosslinking (provide illustrations with text labeis). Include chcmical structures or functionalities for each structure from A to D. Discuss the different polymers physicochemical and biological properties such as charges, hydrophobicities, aqueous solubilities, and biodegradabilities Explanation answer(s) for Q5C: Stion 6 pt): Bloconjugation. Organic Building Blocks Z Gelain Q6A, Choose molecular building blocks (from 'Organic Building Blocks A-Z above), that can undergo rndicai or Insertion polymerization. Write down all of the possible answers in the following blue box. In ardiion, write down the name of the functional group that allows for the polymers to form in the black box Answers: Function Group: o) Chocse molecular building blocks, from the above collection of chemicals (A thru Z), that can form amlde, esier, or ether linkages with the provided starting materials (in the box). Please fill in (QGB the boxes with your answors. Input only one answer per box. Starting Bond Name Reaction Partner Material Circle One) from A-z pt Final Product Structure pt) o amide ester, or ether amide, ester, or ether amide, ester, or ether amide, ester, or ether 9/10

Answer 1

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