Question

The isoelectric point, pI, of the protein soybean trypsin inhibitor is 4.5 , while that of glyceraldehyde-3-phosphate dehydrogenase is 6.55 What is the net charge of soybean trypsin inhibitor at pH 5.1 What is the net charge of glyceraldehyde-3-phosphate dehydrogenase at pH 6.1 The isoelectric point of alanine is 6.01 ;glutamine, 5.65 During paper electrophoresis at pH 4.3 , toward which electrode does alanine migrate? During paper electrophoresis at pH 5.1, toward which electrode does glutamine migrate?

Answer 1

Let's, ie T example, av amino auid e aT NII 2- Co2 H )-1 (inco) e PI net change = t 1 (for se make it aihic (+1) positive R. net chang e Lo, H 2 then, re make it more loapic PHP iu convert to -Nt and emain seme i.e net change negative -1) Lo 2. A P amd Ph PI Pienemam o positive

0 Soyabean tri psininhibitm=15 change at PH 5 (PHPI PI isin mhi negative pif gryceraldchnde 3-phosphate de hy dnro genave6.55 .. Net change at pH 6'1 (pH <P1) = positive 0 PI 61 alanine 6.0L Netlhangent pH 1.3 lphem) =ピsitive so, positive charge& alanine mii iate towands negatlive electro de cathode ·". Net- charge ar pH, 5.1 (pH <P1) = positive Se, positive h as tomin miate toHardn na gati ve electrodafzcathode) ham