Question

3) You can follow the above reaction in a test tube using a dye that turns purple in the presence of the product (the more product that is made, the more purple the solution). You discover an inhibitor of Enzyme A and want to determine if it is competitive or non-competitive inhibitor. You set up the following reactions and measure the amount of purple using a spectrophotometer. Results are below: Absorbance Conditions Test tube 0.63 Enzyme + substrates Inhibitor added 0.02 Inhibitor + 5x substrates 0.23 Inhibitor + 10x substrates0.59 Is this a competitive or non-competitive inhibitor? Explain.

Answer 1

3) Splulten cale mpler t ( The enzym Strare Compleu ezme ubate o002 hebind ta fnzyme tmaie fot binduy e enyme Lubtrate Complei h amuLelwe amant and hne the ente, absarbareaes Sabeta entalon fnzyme - Su shale Copw

<*> vhie temply uste ts mud higho Ganenta/tn, hat a gnealer e enzyme e atnou UeY nhbitoY u arste USing a Ces enzyme- Rubdhvate Gompler th intal value. hiet unlyble in Oce is ie on adive Ste ol the Gnzyme