Question

Name 3. O:o N-o он ONPG A scientist wants to measure if a test subject can digest lactose. This time, she uses 0.100 M ONPG (a harmless lactose alternative), which in the presence of constant (enzyme). forms a yellow product. You allow the reaction to proceed untif the yetlow product is vivid enough to observe, and vou calculate the initial rate. Data Initial rate (not average) 1.64 Mimin 3.66 M/min point ONPG 0200M 0.300 M 0.400 M 0500 м 0 500 M, no enzyme Time until yellow 9.00 x 10 M/min forever a. Rate law with respect to ONPG:Ta Show work below. LMG b. Determine k using this rate law using both trial 1 and trial 2 c. Solve for the initial rates x and y for trials 3 and 4. sote O d. Based on the data, describe the function of the enzyme in the above reaction. e, what is the half-life for the reaction done in trial I? would you expect the half-life to*t.︸ be the same for the other trials? ds or okr Generally, the yellow color is observed after 80.0% consumption of ONPG. Determine the time required in trials 1-4 until the color is observed. Report answers in seconds orn

Answer 1

1o ma Data point ON F Twitial at 1-Gaの se, und 4 219 send 0.300 M 3 D32 sue 169-K- 0-200 K, (0.3m) 3.66 0 201D 40e M in K.(6300)' 3.66 3 66 ir (e) . In tialⓘ pt., initial Hou , cove. D 200 M No half fe wil be rialn a [Al o% s 80-м, CA1 ,